Journal: Advanced Science

Article Title: S100A9‐Targeted Cowpea Mosaic Virus as a Prophylactic and Therapeutic Immunotherapy against Metastatic Breast Cancer and Melanoma

doi: 10.1002/advs.202101796

Figure Lengend Snippet: CPMV particles show immunoprophylaxis in C57BL/6J mice challenged i.v. with B16F10 melanoma or 4T1‐Luc breast cancer cells. a) Schematic and timeline of the B16F10 prophylaxis study. b) Harvested lungs were fixed and imaged before manual tumor counting. c) Quantitative analysis of the number of tumor nodules found on the surface of the lungs. The + sign indicates the mean while the solid horizontal line indicates the median. d) Repeated B16F10 prophylactic immunotherapy study including an H6 peptide only control. The middle line indicates the mean number of tumor nodules. e) H&E images of the harvested lungs. The dark purple spots are indicative of the B16F10 tumor nodules in the lungs. f) Quantitative analysis of the H&E pictures in e). The ratio of tumor cells to total cells within the H&E images were plotted. The images were analyzed using QuPath software. g) Schematic and timeline of the 4T1‐Luc prophylaxis study. h) Harvested lungs were fixed in Bouin's solution before manual tumor counting. The tumor nodules are highlighted by the red arrows. i) Quantitative analysis of the tumor nodules from the lungs in h). All B16F10 experiments had a sample size of n = 4–5 while 4T1‐Luc experiments were accomplished with a sample size of n = 4. All analyses were done by one‐way ANOVA, and significance was deemed as p < 0.05.* = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001, ns = not significant. The image of the mouse is created with BioRender.com.

Article Snippet: Mouse 4T1‐Luc (CRL‐2539‐LUC2) and B16F10 (CRL‐6475) cells were purchased from ATCC.

Techniques: Control, Software

Journal: Advanced Science

Article Title: S100A9‐Targeted Cowpea Mosaic Virus as a Prophylactic and Therapeutic Immunotherapy against Metastatic Breast Cancer and Melanoma

doi: 10.1002/advs.202101796

Figure Lengend Snippet: S100A9‐targeted CPMV immunotherapy against lung metastasis from i.v. injected B16F10 melanoma and 4T1‐Luc breast cancer cells in mice. a) Treatment schedule of the metastatic B16F10 melanoma model using C57BL/6J mice and therapeutic administration of CPMV and CPMV‐H6. b) Quantitative analysis of tumor nodules counted in lungs harvested post‐treatment. c) Treatment schedule of the metastatic 4T1‐Luc breast cancer model using Balb/c mice. d) Quantitative luminescence of the tumors following region of interest (ROI) measurements of the images from (e). e) Luminescent imaging of the 4T1‐Luc tumors taken on the IVIS. The mice were imaged every two days following 150 mg kg −1 i.p. injection of D‐luciferin, and the luminescence was calculated using ROI measurements from the Living Image 3.0 software. One representative image taken on the IVIS on day 9 is shown. For B16F10 experiments an n = 7–12 animals per group and for the 4T1‐Luc experiments an n = 5 animals per group were assigned. Statistical significance was characterized as p < 0.05. All analyses were done by either one or two‐way ANOVA. * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001, ns = not significant. The image of the mouse is created with BioRender.com.

Article Snippet: Mouse 4T1‐Luc (CRL‐2539‐LUC2) and B16F10 (CRL‐6475) cells were purchased from ATCC.

Techniques: Injection, Quantitative Luminescence, Imaging, Software

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: A selective IDO1 inhibitor, KHK2455, improves efficacy of PD-L1 blockade by modulating both innate and adaptive immunity in a mouse melanoma model

doi: 10.1007/s00262-025-04232-8

Figure Lengend Snippet: Abundance of immune cells and tumor inflammation signature in tumors from mice treated with KHK2455, anti–PD-L1 antibody, or their combination. The heatmap displays the cell type scores for each immune cell ( A ) and the expression levels for each gene in the TIS ( C ). Tumor specimens with similar cell type or gene expression profiles were clustered using the Ward’s method. The color scale represents the level of the cell type scores or gene expression, with red indicating higher scores/expression levels. B , D The dots represent individual tumors, and the bars indicate the mean values (n = 7/group). Differences in each cell type score and TIS between the combination and anti–PD-L1 groups were assessed using the Wilcoxon rank-sum test. DC, dendritic cell; NK, natural killer; PD-L1, programmed death-ligand 1; Th1, T-helper cell type 1; TIS, tumor inflammation signature

Article Snippet: A mouse breast cancer cell line, 4T1-Luc2 cells (Caliper Life Sciences), a mouse pancreatic cancer cell line, PAN 02 cells (National Cancer Institute (NCI) Division of Cancer Treatment and Diagnosis (DCTD) Tumor Repository), and a mouse melanoma cell line, B16-F10-Luc cells (gifted from Takashi Murakami, Saitama Medical University), were cultured in RPMI 1640 medium (Life Technologies) supplemented with 10 vol% heat-inactivated fetal bovine serum (FBS) (Life Technologies).

Techniques: Expressing, Gene Expression